Chromium Connect

Plan ahead by referring to the list of equipment, kits, and reagents needed for Single Cell Immune Profiling.

Experimental Design

Experimental Design & Planning

Prepare optimal single cell/nuclei suspension by following our Demonstrated Protocols.

Sample Prep

<Text as={"p"} size={"medium"} style={{ lineHeight: "28px"}} weight={"regular"}>To check if a Demonstrated Protocol is compatible with a specific chemistry version, refer to the <Anchor href={"/support/single-cell-immune-profiling/documentation/steps/sample-prep/compatibility/single-cell-v-d-j-demonstrated-protocol-compatibility-table"}><Text as={"span"} color={"inherit"} size={"medium"} weight={"medium"}>{"Demonstrated Protocol Compatibility Table"}</Text></Anchor><Text as={"span"} size={"medium"} weight={"regular"}>.</Text></Text>

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol.

Library Construction

Use pre-designed or custom panels for targeted gene expression, followed by sequencing and data analysis.

Targeted

Targeted Gene Expression (Optional)

<DocumentCallout><Text as={'p'} size={'medium'}>{'After June 30, 2023, new Cell Ranger and Space Ranger releases will no longer support targeted gene expression analysis.'}</Text></DocumentCallout><Text as={"p"} size={"medium"} style={{ lineHeight: "28px"}} weight={"regular"}>To check if a Demonstrated Protocol is compatible with a specific chemistry version, refer to the <Anchor href={"/support/single-cell-immune-profiling/documentation/steps/targeted-gene-expression/targeted-gene-expression-protocol-compatibility-table"}><Text as={"span"} color={"inherit"} size={"medium"} weight={"medium"}>{"Demonstrated Protocol Compatibility Table"}</Text></Anchor><Text as={"span"} size={"medium"} weight={"regular"}>.</Text></Text>

Sequence your single cell library as per our provided recommendations.

Sequencing

Analyze your single cell data using our pipelines, visualization, and Cloud Analysis tools.

Software Analysis

Chromium X Series

Advanced system for multiomic analysis of hundreds to millions of cells.

Chromium Controller

Compact system for multiomic analysis of hundreds to tens of thousands of single cells.

Automated system for single cell partitioning, barcoding, and library preparation.