Spatial Gene Expression: Resolving the mammalian brain by combining anatomical and sequencing data
Identifying individual cells and their genetic makeup are critical for understanding their roles in how the central nervous system (CNS) physiologically functions, develops, and organizes. Spatial transcriptomics technology integrates histological and sequencing data. Here, we significantly improve performance of spatial transcriptomics with changes to the oligo array, biochemistry, and workflow. These improvements allow for an unbiased clustering of cell type that reliably correlates with the neuroanatomy of both rodent and human specimens.
Identifying individual cells and their genetic makeup are critical for understanding their roles in how the central nervous system (CNS) physiologically functions, develops, and organizes. Spatial transcriptomics technology integrates histological and sequencing data. Here, we significantly improve performance of spatial transcriptomics with changes to the oligo array, biochemistry, and workflow. These improvements allow for an unbiased clustering of cell type that reliably correlates with the neuroanatomy of both rodent and human specimens.