Barcode Enabled Antigen Mapping — Reagent Assembly, Sample Labeling & Flow Sorting
Chromium Single Cell 5’ Barcode Enabled Antigen Mapping (BEAM) enables multiplexed screening of antigen targets to match unique antigens with their corresponding B-cell receptors (BCRs) and T-cell receptors (TCRs), allowing rapid discovery of antigen-specific BCRs (BEAM-Ab) and TCRs (BEAM-T), respectively.
Before starting with the BEAM workflow, appropriate antigens and peptides must be obtained. Chromium Single Cell 5’ BEAM Core Kit, PE, Set A 128 rxns PN-1000539 provides cell labeling and reagent assembly solution through the use of a set of 16 unique BEAM Conjugates, each composed of a streptavidin, a fluorophore molecule (Phycoerythrin, PE), and a Feature Barcode oligonucleotide. The antigens (for BEAM-Ab) or peptides (for BEAM-T) are first assembled with BEAM Conjugates to prepare BEAM-Ab or BEAM-T Assemblies, respectively. BEAM-T Assembly also requires MHC monomers (part of 10x Genomics Chromium Human/Mouse MHC Class I Monomer Kits). These assembled reagents are then used to label individual cells and the labeled cells are sorted through flow cytometry to collect antigen-specific B or T cells prior to loading onto a 10x Genomics chip.
This document provides guidance for preparing BEAM assemblies, cell labeling, and flow sorting for both BEAM-Ab and BEAM-T workflows. Cells prepared using this document can be used as input into the relevant Chromium Single Cell Immune Profiling Solutions User Guide with Feature Barcode technology (see References).
Chromium Single Cell 5’ Barcode Enabled Antigen Mapping (BEAM) enables multiplexed screening of antigen targets to match unique antigens with their corresponding B-cell receptors (BCRs) and T-cell receptors (TCRs), allowing rapid discovery of antigen-specific BCRs (BEAM-Ab) and TCRs (BEAM-T), respectively.
Before starting with the BEAM workflow, appropriate antigens and peptides must be obtained. Chromium Single Cell 5’ BEAM Core Kit, PE, Set A 128 rxns PN-1000539 provides cell labeling and reagent assembly solution through the use of a set of 16 unique BEAM Conjugates, each composed of a streptavidin, a fluorophore molecule (Phycoerythrin, PE), and a Feature Barcode oligonucleotide. The antigens (for BEAM-Ab) or peptides (for BEAM-T) are first assembled with BEAM Conjugates to prepare BEAM-Ab or BEAM-T Assemblies, respectively. BEAM-T Assembly also requires MHC monomers (part of 10x Genomics Chromium Human/Mouse MHC Class I Monomer Kits). These assembled reagents are then used to label individual cells and the labeled cells are sorted through flow cytometry to collect antigen-specific B or T cells prior to loading onto a 10x Genomics chip.
This document provides guidance for preparing BEAM assemblies, cell labeling, and flow sorting for both BEAM-Ab and BEAM-T workflows. Cells prepared using this document can be used as input into the relevant Chromium Single Cell Immune Profiling Solutions User Guide with Feature Barcode technology (see References).