Direct capture of guide RNAs enables scalable and combinatorial single cell CRISPR screens
Researchers at the University of California, San Francisco (San Francisco, CA, USA), with scientists from 10x Genomics, described a method to streamline CRISPR screens by directly capturing and sequencing guide RNAs in single cells, without the need for a separate indexing transcript. Direct capture of guide RNAs enables accurate identification at the single cell level, and opens the door for researchers to test combinatorial CRISPR perturbations and detect the resulting cell phenotypes. This scalable method to simultaneously capture and analyze guide RNAs, together with their effect on the transcriptome, is enabled with Chromium Single Cell Gene Expression with Feature Barcode technology.
Researchers at the University of California, San Francisco (San Francisco, CA, USA), with scientists from 10x Genomics, described a method to streamline CRISPR screens by directly capturing and sequencing guide RNAs in single cells, without the need for a separate indexing transcript. Direct capture of guide RNAs enables accurate identification at the single cell level, and opens the door for researchers to test combinatorial CRISPR perturbations and detect the resulting cell phenotypes. This scalable method to simultaneously capture and analyze guide RNAs, together with their effect on the transcriptome, is enabled with Chromium Single Cell Gene Expression with Feature Barcode technology.