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Massively parallel simultaneous profiling of the transcriptomic and epigenomic landscape at single cell resolution

Massively parallel simultaneous profiling of the transcriptomic and epigenomic landscape at single cell resolution

We present here a single cell high-throughput assay to simultaneously profile 3' gene expression and open chromatin via ATAC-seq from the same nucleus. Simultaneous profiling allows for finer resolution of cell types in terminally differentiated cells and cells on a developmental trajectory. This assay has the potential to reveal the complex interplay between regulatory elements, open chromatin, and gene expression.

We present here a single cell high-throughput assay to simultaneously profile 3' gene expression and open chromatin via ATAC-seq from the same nucleus. Simultaneous profiling allows for finer resolution of cell types in terminally differentiated cells and cells on a developmental trajectory. This assay has the potential to reveal the complex interplay between regulatory elements, open chromatin, and gene expression.